Product introduction

 

filmlet Magnetic Separation

 

Magnetic Bead Troubleshooting

 

BILATEST® Genomic DNA Kits

 

BILATEST® RNA Kits

 

BILATEST® Plasmid Kit

 

BILATEST® PCR Cleanup Kit

 

BILATEST® Sequence Cleanup Kit

 

 

 

 

Magnetic stands

 

Stands for 96-well plates

 

Stands for tubes

 

Molecular Weight Markers

 

 

BILATEST® magnetic particle kits

 

The fast and simple solution for sample preparation.

 

The principle of magnetic bead separation

 

The BILATEST® kits utilize proprietary Magnetic Bead technology to isolate nucleic acids with both high yield and purity.

Their scalability together with protocols and equipment facilitate quick preparations from very small to large amounts of sample material. The removal of conventional spin steps from kit protocols, the high magnetite content of the beads as well as the easy handling make these kits highly adaptable to automation but also for manual use. A general scheme for the isolation process is shown.

 

 

 


 

BILATEST® Genomic Kits

 

BILATEST® Genomic DNA 10 Kit

 

BILATEST® Genomic DNA 100 Kit

 

BILATEST® Genomic DNA 250 Kit

 

BILATEST® DNA Food Kit

 

BILATEST® Bacteria Kit

 

BILATEST® DNA Tissue Kit

 

BILATEST® Plant Kit

 

 

BILATEST® Genomic DNA 10 Kit

 

BILATEST® Genomic DNA 10 kit is designed for fast and easy isolation of PCR-ready genomic DNA from 10µl whole blood. Coated magnetic beads with a polymeric surface for DNA-binding prevent any leakage of iron from the magnetic core.

 

The complete protocol takes approximately 30 minutes, the expected yield from 10µl whole blood from healthy persons is about 0.2-0.3 µg DNA.

 

The kit includes a lysis buffer optimized for the lysis of blood cells.

 

Application: Extraction of cellular genomic DNA

 

Sample volume: 1 - 20 µl

 

Material: fresh or frozen human- or animal blood (Citrate-, EDTA-, or Heparin-stabilised); plasma, serum, buffy coat, saliva

 

Preparation time: 15 – 20 min

 

Rate of yield: 0.2 - 0.4 µg

 

A260/A280: 1.70 - 1.95

 

Manual

 

 

 

 

Description

Genomic DNA 10 Kit

Genomic DNA 10 Kit

Cat.No.

110102

110103

Isolations

100

1000

Scale

96-well plate

96-well plate

 

 

 

BILATEST® Genomic DNA 100 Kit

 

BILATEST® Genomic DNA 100 kit is designed for fast and easy isolation of PCR-ready genomic DNA from 100µl whole blood. Coated magnetic beads with a polymeric surface for DNA-binding prevent any leakage of iron from the magnetic core.

 

The complete protocol takes approximately 30 minutes, the expected yield from 100µl whole blood from healthy persons is about 2 - 4 µg DNA.

 

The kit includes a lysis buffer optimized for the lysis of blood cells.

 

Application: Extraction of cellular genomic DNA

 

Sample volume: 100 - 200 µl

 

Material: fresh or frozen human- or animal blood (Citrate-, EDTA-, or Heparin-stabilised); plasma, serum, buffy coat, saliva

 

Preparation time: 25 – 35 min

 

Rate of yield:2 - 4 µg

 

A260/A280:1.70 - 1.95

 

Manual

 

 

 

Description

Genomic DNA 100 Kit

Genomic DNA 100 Kit

Cat.No.

110202

110203

Isolations

100

1000

Scale

1.5/2 ml tubes

1.5/2 ml tubes

 

 

 

BILATEST® Genomic DNA 250 Kit

 

BILATEST® Genomic DNA 250 kit is designed for fast and easy isolation of PCR-ready genomic DNA from 250µl whole blood. Coated magnetic beads with a polymeric surface for DNA-binding prevent any leakage of iron from the magnetic core.

 

The complete protocol takes approximately 30 minutes, the expected yield from 250µl whole blood from healthy persons is about 5 - 10 µg DNA.

 

The kit includes a lysis buffer optimized for the lysis of blood cells.

 

Application: Extraction of cellular genomic DNA

 

Sample volume: 250 µl

 

Material: fresh or frozen human- or animal blood (Citrate-, EDTA-, or Heparin-stabilised); plasma, serum, buffy coat, saliva

 

Preparation time: ca. 30 min

 

Rate of yield:5 - 10 µg

 

A260/A280:1.70 - 1.95

 

Manual

 

 

 

Description

Genomic DNA 250 Kit

Genomic DNA 250 Kit

Cat.No.

111402

111403

Isolations

50

1000

Scale

1.5/2 ml tubes

1.5/2 ml tubes

 

 

 

BILATEST® DNA Tissue Kit

 

BILATEST® DNA Tissue Kit is designed for DNA purification from different kinds of tissues.

The kit includes a lysis buffer optimized for the lysis of tissues.

DNA which is released during lysis binds to the magnetic particles. After some washing steps according to the protocol the DNA is eluted from the magnetic particles for PCR.

The expected yield from 10mg mouse tail tissue is about 15-25µg DNA and about 24-40µg DNA from 10mg liver tissue.

 

Application: Isolation of genomic DNA from different tissue materials

 

Sample volume:10 mg tissue, 2-4 mm mouse tail pieces

 

Material: animal tissue or cells (mouse tails, liver etc.) gram-negative bacteria

 

Preparation time: 25 – 30 min (without lysis)

 

Rate of yield: 15 - 25 µg (2-4 mm mouse tail) 24 – 40 µg (10 mg liver)

 

A260/A280: 1.70 - 1.95

 

Manual

 

 

 

Description

DNA Tissue Kit

DNA Tissue Kit

Cat.No.

110402

110403

Isolations

100

1000

 

 

 

BILATEST® DNA Food Kit

 

The BILATEST® DNA Food Kit is designed for DNA purification from food and plant materials.

DNA which is released during lysis binds to the magnetic particles. After some washing steps according to the protocol the DNA is eluted from the magnetic particles.

The eluted DNA or the bound DNA-magnetic-particle-complex without elution can be used for PCR.

The expected yield from 200mg food is about 0.1-10µg DNA depending on the sample material

 

Application: Extraction of DNA from different food samples

 

Sample volume: 200 mg food samples

 

Material: Food samples (mostly vegetable origin)

 

Preparation time: 60 – 70 min

 

Rate of yield:  0.1 - 10 µg (sample dependent)

 

A260/A280: 1.70 - 1.95

 

Manual

 

 

 

Description

DNA Food Kit

DNA Food Kit

Cat.No.

110602

110603

Isolations

100

1000

 

 

 

BILATEST® Bacteria Kit

 

The BILATEST® Bacteria Kit is designed for the fast and easy isolation of genomic DNA from bacteria.

The kit is particularly suitable for isolation of DNA from 200 µl culture medium or bacterial pellets.

The kit is suitable for Gram-positive micro organisms as well as for Gram-negative ones and achieves high yields of purified bacterial DNA in very short time.

 

Application: Isolation of genomic DNA from culture medium or bacterial pellets

 

Sample volume:  100 µl culture medium bacteria pellet of 100 – 200 µl medium

 

Material: gram-positive or gram-negative micro organisms

 

Preparation time: 30 min

 

Rate of yield: 2 - 6 µg (sample dependent)

 

A260/A280: 1.70 - 1.95

 

Manual

 

 

 

 

Description

Bacteria Kit

Bacteria Kit

Cat.No.

111002

111003

Isolations

100

1000

 

 

 

BILATEST® Plant Kit

 

BILATEST® Plant Kit is designed fort the purification of total DNA from plant tissues. The protocol can be performed manually.

 

An advantage of an automated procedure is the incorporated removal of grinding beads (steel or tungsten beads) after homogenizing the plant material in a bead mill (e.g. Geno/GrinderTM 2000 or Retsch Mixer Mill MM 300).

 

 

Application: Isolation of total DNA from plant tissue

 

Sample volume: 20 - 50 mg tissue

 

Sample material: plant tissue

 

Preparation time: 50 min. (manually)

 

Rate of yield: 5-30 µg/20 mg tissue

 

Manual

 

 

 

 

Description

Plant Kit

Plant Kit

Cat.No.

111202

111203

Isolations

100

1000

 

 

 

BILATEST® RNA Kits

 

BILATEST® Viral RNA/DNA Kit

 

BILATEST® Isodirect mRNA Kit

 

 

BILATEST® Viral RNA/DNA Kit

 

BILATEST® Viral RNA/DNA Kit is designed for purification of viral RNA and DNA from serum, plasma or whole blood.

 

Viral RNA or DNA which is released during lysis binds to the magnetic particles. After some washing steps according to the protocol DNA and RNA are eluted from the magnetic particles.

Eluted DNA or RNA is then ready for PCR.

 

The detection limit for HBV is about 40 copies/ ml from serum or plasma.

 

Application: parallel isolation of viral RNA and DNA

 

Sample volume: 200 µl

 

Material:  serum, plasma, blood

 

Preparation time: 45 min

 

Sensitivity: 40 copies/ml

 

Manual

 

 

 

 

Description

Viral RNA/DNA Kit

Viral RNA/DNA Kit

Cat.No.

110402

110403

Isolations

100

1000

 

 

BILATEST® Isodirect mRNA Kit

 

The BILATEST® Isodirect mRNA Kit provides direct extraction of mRNA from crude extracts of animal tissues, cells, plants or blood.

 

Isolation of mRNA will be done by base pairing of the poly A tail of messenger RNA to oligo dT sequence bound to magnetic beads surface.

 

The high stability of the magnetic beads allows regeneration of beads for reuse.

 

The kit assures high yields of purified mRNA. The extracted mRNA is suitable for a lot of different applications (RT-PCR etc.).

Application: direct Isolation of mRNA

 

Sample volume: 107 (cells), 100 mg (tissue)

 

Material: cell lines, animal tissue, plant tissue

 

Preparation time: 20 - 25 min

 

Rate of yield:  2 µg

 

Manual

 

 

 

 

Description

Isodirect mRNA Kit

Isodirect mRNA Kit

Cat.No.

110901

110903

Isolations

50

1000

 

 

BILATEST® Plasmid Mini Kit

 

The BILATEST® Plasmid Mini Kit is designed for fast and easy isolation of plasmid DNA from bacteria.

 

Coated magnetic beads with a polymeric surface for DNA-binding prevent any leakage of iron from the magnetic core.

 

The complete protocol takes approximately 20 minutes, the expected yield from 1.5ml overnight bacteria culture (DH-5-α E. Coli) is about 3-8 µg high copy plasmid-DNA (pUC-18).

 

Application: Isolation of Plasmid-DNA

 

Sample volume: 1 – 3 ml

 

Material: bacteria medium

 

Preparation time: 30 min

 

Rate of yield: up to 8 µg (from 1.5 ml bacteria culture)

 

A260/A280: 1.80 - 1.95

 

Manual

 

 

 

Description

Plasmid Mini Kit

Plasmid Mini Kit

Cat.No.

110302

110303

Isolations

100

1000

 

 

 

BILATEST® PCR Cleanup Kit

 

The BILATEST® PCR Cleanup Kit is designed to purify PCR products by removing impurities while maintaining a high yield of PCR product.

 

The protocol is scalable for purification from starting volumes from 50 µl up to a few millilitres of PCR samples.

 

PCR products larger than 100 bp in size have been purified. Purified PCR fragments are eluted in either a low salt buffer or water and can be used for microarrays, automated fluorescent DNA sequencing and restriction digestion.

 

Application: Purification of PCR-products

 

Sample volume: 50 µl – a few ml

 

Material: PCR-reactions

 

Preparation time: 25 min

 

Recovery: ca. 95%.

 

Manual

 

 

 

 

Description

PCR Cleanup Kit

PCR Cleanup Kit

Cat.No.

111302

111303

Isolations

100

1000

 

 

BILATEST® Sequence Cleanup Kit

 

The BILATEST® Sequence Cleanup Kit is designed for purifying sequencing reactions.

The clean-up process requires no centrifugation or filtration steps and removes any type of dye terminator from 5 – 20 μl sequencing reactions. The obtained results are of the highest quality with high fluorescent signal intensities and routinely long read lengths (> 900 bp).

 

Application: Purification of sequencing reactions

 

Sample volume: 5 - 20 µl

 

Material: sequencing reactions

 

Preparation time: 25 min

 

Recovery: ca. 95%.

 

Manual

 

 

 

Description

Sequence Cleanup Kit

Sequence Cleanup Kit

Cat.No.

110802

110803

Isolations

100

1000

 

 

GENPOINT KITS

 

GENPOINT BUGS’N BEADS Kit Version S/C (with Standard or Clinical beads)

 

The BUGS'N BEADS™ kit extracts bacterial DNA from complex solutions like clinical and food samples, removing PCR inhibiting components found in these substances.

Kit is available with Standard (S) beads and Clinical (C) beads depending on desired bacterial strain.

The uniquely coated magnetic beads allow for the immobilisation of bacteria on the particles, and after lysis, the bacterial DNA is immobilised on the same particles. This allows the whole assay to take place in just one tube, prior to PCR analysis of the DNA.

 

 

Application: Isolation of pathogen bacteria-DNA

 

Sample volume: 100µl

 

Material: blood, blood products, spinal fluid, urine, faeces, dairy products, meat & poultry, fish, fruits & vegetables, legumes & nuts

 

Preparation time: ca. 35 min

 

Rate of yield: bacteria DNA for ca. 5 – 10 PCR reactions

 

Manual

 

 

 

Description

BUGS’N BEADS S

BUGS’N BEADS C

Cat.No.

GK1-100 S

GK2-100 C

Isolations

100

100

 

 

GENPOINT BUGS’N BEADS Kit Version U (with “Urogenital” beads)

 

The BUGS'N BEADS™ kit version U (with “Urogenital” beads) for fast and easy DNA purification from bacterial strains associated with respiratory and genital and urogenital related diseases like:

 

·          Streptococcus

·          Staphylococcus

·          Chlamydia spp.

·          Neisseria

·          Listeria

·          Mycobacterium

·          Mycoplasma spp

·          Bacillus

 

 

Application: Isolation of DNA from bacterial strains

 

Sample volume: 100µl

 

Material: variety of matrixes associated with respiratory, genital and urogenital related diseases

 

Preparation time: ca. 30 min

 

Rate of yield: bacteria DNA for ca. 5 – 10 PCR reactions

 

Manual

 

 

 

Description

BUGS’N BEADS U

Cat.No.

GK1-100 U

Isolations

100

 

 

 

GENPOINT BUG TRAP Kit

 

Following extraction of bacteria with the BUG TRAP kit, the bacteria can be subjected to a wide variety of applications such as antibody-based detection and ATP-measurements, or inoculation into specific medium for further enrichment.

Application: Isolation of living bacteria

 

Sample volume: 100µl

 

Material: blood, blood products, spinal fluid, urine, faeces, dairy products, meat & poultry, fish, fruits & vegetables, legumes & nuts

 

Preparation time: ca. 25 min

 

Manual

 

 

 

Description

BUG TRAP KIT S

BUG TRAP KIT C

Cat.No.

BT1-100 S

BT2-100 C

Isolations

100

100

 

 

GENPOINT PCR Primer

 

DNA sequences are currently providing the most precise information available for identification of an organism. By utilizing DNA sequences from the desired micro organisms, GENPOINT has developed primers that are highly specific for some of the most important pathogenic bacteria, including Chlamydia trachomatis, Campylobacter spp., Campylobacter jejuni, Campylobacter coli, Listeria spp., Listeria monocytogenes, Salmonella spp, Staphylococcus aureus, Bacillus cereus, and E. coli O157.

 

Primer Overview

 

 

 

 

 

 Cat.no.

Genpoint general purpose primer pairs

 Amount

 

 

 

110-PSA

Primer pair Staphylococcus Aureus

(2x 2000 pmol)

110-PC

Primer pair Campylobacter spp

(2x 2000 pmol)

110-PCJ

Primer pair Campylobacter jejuni

(2x 2000 pmol)

110-PCC

Primer pair Campylobacter coli

(2x 2000 pmol)

110-PL

Primer pair Listeria spp.

(2x 2000 pmol)

110-PLM

Primer pair Listeria monocytogenes

(2x 2000 pmol)

110-PS

Primer pair Salmonella spp.

(2x 2000 pmol)

110-PEC

Primer pair O157 E.coli

(2x 2000 pmol)

110-PVC

Primer pair Vibrio cholerae

(2x 2000 pmol)

110-PYV

Primer pair Yersinia virulens

(2x 2000 pmol)

110-PBC

Primer pair Bacillus cereus

(2x 2000 pmol)

110-PCCT

Primer pair Chlamydia

(2x 2000 pmol)

 

10x  Primer pair (2x 2000 pmol) each

10x (2x 2000 pmol)

 

 Cat.no.

Genpoint research only primer pairs

 Amount

 

 

 

110-PAE

Primer pair Aeromonas spp.

(2x 2000 pmol)

110-PBB

Primer pair Borrelia

(2x 2000 pmol)

110-PCM

Primer pair Corynebacterium spp.

(2x 2000 pmol)

110-PEH

Primer pair E.coli EHEC

(2x 2000 pmol)

110-PIA

Primer pair E. coli, Intimin Alfa

(2x 2000 pmol)

110-PIB

Primer pair E. coli, Intimin Beta

(2x 2000 pmol)

110-PIG

Primer pair E. coli, Intimin Gamma

(2x 2000 pmol)

110-PIG2

Primer pair E. coli, Intimin Gamma2

(2x 2000 pmol)

110-PEI

Primer pair E. coli, EIEC-Shigella spp.

(2x 2000 pmol)

110-PES

Primer pair E. coli - Shigella spp.

(2x 2000 pmol)

110-PEN

Primer pair Enterobacter

(2x 2000 pmol)

110-PMT1

Primer pair Mycobact. tub. Complex

(2x 2000 pmol)

110-PMT2

Primer pair Mycobact. tub. Complex2

(2x 2000 pmol)

110-PMP

Primer pair Mycobact. paratub.

(2x 2000 pmol)

110-PMG

Primer pair Mycoplasma genitalium

(2x 2000 pmol)

110-PNG

Primer pair Neisseria

(2x 2000 pmol)

110-PPA

Primer pair Pseudomonas spp.

(2x 2000 pmol)

110-PPR

Primer pair Propionebacterium

(2x 2000 pmol)

110-PRS

Primer pair Rhodococcus

(2x 2000 pmol)

110-PSE

Primer pair Stahylococcus epidermidis

(2x 2000 pmol)

110-PSM

Primer pair Staph. aur. – epidermidis

(2x 2000 pmol)

110-PST

Primer pair Streptococcus spp.

(2x 2000 pmol)

110-PU

Primer pair Ureaplasma

(2x 2000 pmol)

110-PYV

Primer pair Yersinia vir. plasmid

(2x 2000 pmol)

110-PYE

Primer pair Yersinia enterocolitica

(2x 2000 pmol)

 

GENPOINT POWERlyse™

 

The new POWERlyse™ solution is an extremely efficient reagent, developed especially for bacteria such as Staphylococcus sp., Streptococcus sp, Mycobacterium sp., and Chlamydia trachomatis elementary bodies. One minute incubation at room temperature is all that is required to obtain 100% lysis.

 

POWERlyse™ must be used in conjunction with DNA isolation system using magnetic particles or other solid phase systems to bind the DNA, since it is not directly compatible with nucleic acid amplification and must be removed prior to analysis.

 

POWERlyse™ is included in all Genpoint's magnetic particle based DNA isolation kits (BUGS'N BEADS™ and chlamCAP). POWERlyse™ may also replace the lysis reagents accompanying other DNA isolations whenever working with bacteria tough to break.

 

Manual

 

 

 

 

Description

POWERlyse™ 100x

POWERlyse™ 1000x

Cat.No.

PLB-100

PLB-1000

 

5 ml

50 ml

 

 

 

BILATEST® Magnetic stands for BILATEST® magnetic particle kits

The BILATEST® magnetic stands are designed to guarantee optimal results in combination with BILATEST® magnetic particle kits. Naturally BILATEST® stands could also be used with each other kit on the basis of magnetic particles.

 

BILATEST® magnetic stands consist out of variably sized transparent acrylic glass (Plexiglasâ) blocks allowing users permanent optical control of magnetic separation.

 

All used magnets are durable, extra strong permanent magnets which guarantee a very fast magnetic separation in varying sample volumes.

 

Bilatec offers customers a broad range of magnetic stands for different sample- and elution volumes and applications. The assortment ranges from the “normal” Bilatest magnetic separator M96 for applications in 96 well plate format, M12+12 stands for 1.5ml/ 2.0ml tubes, M96 deep well plate separators up to XXL stands for 5-50ml applications.

 

Furthermore it is possible to adapt Bilatest magnetic separators in addition to manual use to different robotic systems (with SBS standard format). Thus the manual magnetic separator M96 is also available as adaptation for JobiWell robotic system from Cybio AG/ Jena and for RoboAmp and RoboSeq robotic systems from MWG Biotech/Aviso GmbH. In cooperation with you it is also possible to design magnetic stands for your requirements. Please request for individual solutions.

 

The BILATEST® magnetic separators:

 

BILATEST®  separator M96 for magnetic separations in 96 well plates

 

BILATEST®  separator M96 for Small Volume for small elution volumes in 96 well plates

 

BILATEST®  separator M96 MWG and BILATEST®  separator M96 for Small Volume MWG for the use with MWG RoboAmp and RoboSeq robotic systems.

 

BILATEST®  separator M12+12 for magnetic separations in 12x 1.5ml and 12x 2.0ml tubes and BILATEST®  separator M2 for magnetic separations in 2x 1.5ml  or 2x 2.0ml tubes.

 

BILATEST®  separator Deep Well for magnetic separations in Deep Well plates.

 

BILATEST®  separator XXL for magnetic separations in big volumes up to 50ml volume in 50ml tubes.

 

 

 

Separators for 96-well plates

 

BILATESTâ M96

BILATESTâ M96 Small volume

BILATESTâ M96 Deep Well Standard

BILATESTâ M96 Deep Well TS

 

 

BILATESTâ M96

 

Consists of a clear Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 7.5 mm) for fast magnetic separation with 96 well plates.

 

Optimized for BILATEST® Kits and THERMOSPRINT®-96 well plates.

 

For fast separation (< 2 min).

 

For 96 well plates with SBS Standard-Format.

 

BILATESTâ M96 Small volume

 

Ideal for DNA elution in small volumes                    (< 20 µl).

 

Consists of a clear Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 9.5 mm) for fast separation with 96 well plates.

 

Optimized for BILATEST® Kits and THERMOSPRINT®-96 well plates.

 

For fast separation (< 2 min).

 

For 96 well plates with SBS Standard-Format.

 

 

 

Description          

Bilatest M96

Bilatest M96 for Small Volume

Cat.No

209601

209606

Format

96 well plates

96 well plates

Material

Plexiglas®

Plexiglas®

Colour

transparent

transparent

Item/pack

1

1

Specification

for elution volume > 20µl

for elution volume < 20µl

 

 

BILATESTâ M96 Deep Well Standard

 

Ideal for DNA purification using standard 2ml Deep Well plates.

 

Consists of a clear Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 9.5 mm) for fast separation.

 

Optimized for the use of BILATEST® Kits.

 

For fast separation (< 3 min).

 

 

BILATESTâ M96 Deep Well TS

 

Ideal for DNA purification using 2ml Deep Well plates with pyramidal bottom.

 

Consists of a clear Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 7.5 mm) for fast separation.

 

Optimized for the use of BILATEST® Kits.

 

For fast separation (< 3 min).

 

For 96 well plates in SBS Standard-Format.

 

Description

Bilatest M96 Deep Well

Bilatest M96 Deep Well TS

Cat.No.

209618

209618-TS

Format

96 deep well Plates

96 deep well Plates

Material

Plexiglas®

Plexiglas®

Colour

transparent

transparent

Item/pack

1

1

Specifications

for Standard Deep Well plates

Deep Well plates with pyramidal bottoms

 

 

 

Separators for tubes

 

BILATESTâ M 12 + 12

BILATESTâ M 2

BILATESTâ Extension-Module M20

for Separator M96 and M96 Small Volume

 

BILATESTâ XXL

 

BILATESTâ M96 MWG

BILATESTâ M96 MWG for Small Volume

 

 

BILATESTâ M 12 + 12

 

Consists of a clear Plexiglasâ block with 12x extra strong permanent magnets for a fast magnetic separation with 1.5ml and 2.0ml tubes.

 

Optimized for the use of BILATEST® Kits.

 

With 48 tube positions including 24 positions for magnetic separation (12x for 1.5ml and 12x for 2.0ml tubes).

 

For 1.5ml (conical) and 2.0ml tubes (conical recommended).

 

Transparent material allows constant visual control of the separation.

 

For fast separation (< 2 min).

 

 

BILATESTâ M 2

 

Consists of a aluminium block with 2x extra strong permanent magnets for a fast magnetic separation with 1.5ml or 2.0ml tubes.

 

Optimized for the use of BILATEST® Kits.

 

The design allows a constant visual control of the separation.

 

For fast separation (< 2 min).

 

 

Description

Bilatest M12+12

Bilatest M 2

Cat.No.

210141

209600

Format

1,5/ 2ml tubes

1,5/ 2ml tubes

Material

Plexiglas®

Aluminium

Colour

transparent

Aluminium

Item/pack

1

1

Specifications

for 12x 1.5 and 2ml tubes

for 2x 1.5 or 2ml tubes

 

 

 

BILATESTâ Extension-Module M20 for Separators M96 and M96 Small Volume

 

The Extension Module M20 allows manual magnetic  separation in 1.5ml microtubes on the BILATEST®  Separator M96 or BILATEST®  Separator M96 for small volumes, thus replacing BILATEST® Separator M12+12 for 1.5 ml and 2.0 ml tubes. 

 

We recommend this accessory module for occasional separations with 1.5ml microtubes. For frequent use with 1.5ml microtubes we recommend the special BILATEST® Separator M12+12 for 1.5 ml and 2.0 ml tubes.

 

The magnetic separator consists of transparent material which allows visual monitoring of the magnetic separation.

 

Makes it possible to transfer up to 20 tubes at a time onto the BILATEST Separator M96.

 

For optimum magnetic separation in small elution volumes use BILATEST Separator M96 for small volumes.

 

 

Description

Extension-Module M20

Cat.No.

209602

Format

1,5 – 2.0ml Tubes

Material

Plexiglas®/ PS

Colour

transparent

Item/pack

1

Specifications

For magnetic separation of tubes on a 96-well magnetic stand

 

 

BILATESTâ XXL

Type 1

 

Magnetic separator for manual separation using BILATEST® Kits in high volumes.

 

Consists of a clear Plexiglasâ block with 2 holders for 50ml tubes. One duct with one magnet for separation from a maximum 25ml volume and one with two magnets for separation of a maximum 50ml liquid volume.

 

Optimized for use with 50ml tubes and BILATEST® Kits or other kits using magnetic particles.

 

Transparent material allows constant visual control of the separation.

 

 

Type 2

 

Magnetic separator for manual separation using BILATEST® Kits in high volumes.

 

Consists of a clear Plexiglasâ block with 2 holders for 50ml tubes. Each duct has one magnet for separation from a maximum 25ml liquid volume.

 

Optimized for use with 50ml tubes and BILATEST® Kits or other kits using magnetic particles.

 

Transparent material allows constant visual control of the separation.

 

 

Description

Bilatest XXL Type 1

Bilatest XXL Type 2

Cat.No.

209616

209617

Format

2x 50ml tubes

2x 50ml tubes

Material

Plexiglas®

Plexiglas®

Colour

transparent

transparent

Item/pack

1

1

Specifications

Magnetic separation up to 50ml volume

Magnetic separation up to 25ml volume

 

 

BILATESTâ M96 MWG

 

Magnetic separator for adaptation of the BILATEST®- magnetic particle technology on a RoboAmp or Roboseq robotic system (MWG).

 

Consists of a Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 7.5 mm) for fast separation using 96 well plates.

 

Optimized for use with BILATEST® Kits and THERMOSPRINT®-96 well plates.

 

For 96 well plates with SBS standard format.

 

 

BILATESTâ M96 MWG for Small Volume

 

Magnetic separator for adaptation of the BILATEST®- magnetic particle technology on a RoboAmp or Roboseq pipetting robot (MWG).

 

Ideal for DNA elution in small volumes (< 20 µl).

 

Consists of a clear Plexiglasâ block with 6 x 4 extra strong permanent magnets (d = 9.5 mm) for fast separation with 96 well plates.

 

Optimized for use with BILATEST® Kits and THERMOSPRINT®-96 well plates.

 

For 96 well plates with SBS standard format.

 

 

Description

Bilatest M96 MWG

Bilatest M96 MWG for Small Volume

Cat.No.

209700

209701

Format

96 well plates

96 well plates

Material

Plexiglas®

Plexiglas®

Colour

transparent

transparent

Item/pack

1

1

Specifications

for elution volume > 20µl

for elution volume < 20µl

 

 

BILATEST® Molecular Weight Markers (MWM)

 

Overview BILATEC Molecular Weight Markers (MWM):

 

BILATEST® Molecular Weight Marker-1 (MWM-1)

 

The BILATEST® Molecular Weight Marker-1 contains 14 regularly-spaced bands ranging from 200bp to 10Kb.

Each band contains an exact amount of dsDNA, enabling accurate quantification.

Bands at 1Kb and 10Kb have the highest intensity for easy identification and orientation. Supplied in a ready-to-load format saving considerable time.

 

 

Application: Quantification of double stranded DNA on gels

 

Size: 200 bp – 10.000 bp

 

Product attributes: each band contains an exact amount of dsDNA (720 ng of DNA / 5 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

Description

MWM-1

MWM-1

Cat.No.

120101

120102

lanes

200

500

 

 

BILATEST® Molecular Weight Marker-2 (MWM-2)

 

The BILATEST® Molecular Weight Marker-2 contains 15 regularly-spaced bands ranging from 50bp to 2Kb.

Each band contains an exact amount of DNA, enabling accurate quantification.

Bands at 300bp, 1Kb and 2Kb have the highest intensity for easy identification and orientation. Supplied in a ready-to-load format saving considerable time.

 

Application: Quantification of double stranded DNA on gels

 

Size: 50 bp – 2000 bp

 

Product attributes: each band contains an exact amount of dsDNA (600 ng of DNA / 5 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

 

Description

MWM-2

MWM-2

Cat.No.

120201

120202

lanes

200

500

 

 

 

BILATEST® Molecular Weight Marker-3 (MWM-3)

 

The BILATEST® Molecular Weight Marker-3 contains 11 bands ranging from 500bp to 5Kb.

Each band contains an exact amount of dsDNA, enabling accurate quantification.

The 1Kb and 3.5Kb bands have the highest intensity for easy identification.

Contains Red dye, migrating at 500bp in 1.5% agarose gel.

Supplied in a ready-to-load format saving considerable time.

 

 

 

 

 

 

 

 

 

Application: Quantification of double stranded DNA on gels

 

Size: 500 bp – 5 Kb

 

Product attributes: each band contains an exact amount of dsDNA (620 ng of DNA / 5 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

 

Description

MWM-3

MWM-3

Cat.No.

120301

120302

lanes

200

500

 

 

BILATEST® Molecular Weight Marker-4 (MWM-4)

 

The BILATEST® Molecular Weight Marker-4 contains 10 regularly-spaced bands ranging from 100bp to 1Kb.

Each band contains an exact amount of DNA, enabling accurate quantification.

Band at 1000 bp has the highest intensity for easy identification.

Supplied in a ready-to-load format saving considerable time.

 

Application: Quantification of double stranded DNA on gels

 

Size: 100 bp – 1 Kb

 

Product attributes: each band contains an exact amount of dsDNA (500 ng of DNA / 5 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

Description

MWM-4

MWM-4

Cat.No.

120401

120402

lanes

200

500

 

 

 

BILATEST® Molecular Weight Marker-5 (MWM-5)

 

The BILATEST® Molecular Weight Marker-5 contains 12 regularly-spaced bands ranging from 25 bp to 500 bp.

Each band contains an exact amount of DNA, enabling accurate quantification.

Bands at 100 bp and 200 bp have the highest intensity for easy identification.

Supplied in a ready-to-load format saving considerable time.

 

Application: Quantification of double stranded DNA on gels

 

Size: 25 bp – 500 bp

 

Product attributes: each band contains an exact amount of dsDNA (960 ng of DNA / 5 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

Description

MWM-5

MWM-5

Cat.No.

120501

120502

lanes

200

500

 

 

BILATEST® Molecular Weight Marker-6 (MWM-6)

 

The BILATEST® Molecular Weight Marker-6 contains 10 bands ranging from 10.09Kb to 48.5Kb.

Each band contains an exact amount of DNA, enabling accurate quantification.

Band at 38420bp has highest intensity for easy identification.

Supplied in a ready-to-load format saving considerable time.

 

Application: Quantification of double stranded DNA on gels

 

Size: 10.09 Kb – 48.5 Kb

 

Product attributes: each band contains an exact amount of dsDNA (830 ng of DNA / 10 µl lane), ready-to-load format minimises the time spent thawing, diluting and adding tracking dye to the DNA Ladder, a 5x sample loading buffer is supplied for your convenience

 

 

 

Description

MWM-6

MWM-6

Cat.No.

120601

120602

lanes

200

500